In 2016 the sixth round ofthe Integrated Biological and Behavioral Surveillance (IBBS) survey was carried out by Intrepid Nepal (INPL) under the leadership of the National Center for AIDS and STD Control (NCASC) and in partnership with Save the Children Nepal. This survey was conducted in line with the objectives of the previous rounds of IBBS surveys; i.e. to determine the prevalence of HIV and STIs, assess HIV and STI related risk behaviors, and explore the level of awareness and understanding of HIV/STIs. This was a descriptive serial cross-sectional study conducted among the female sex workers of 22 highway districts. About 0.8 percent of the female sex workers were found to be HIV positive, whereas the active syphilis was diagnosed positive in 10.3 percent of the FSWs.